Part:BBa_K1458005

Human DsbA-L (GSTK1) gene

DsbA-L is a protein present in the ER (endoplasmic reticulum). Its main action is a Glutathione S-Transferase action on the hormone adiponectin. It has the ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification, and it helps with binding two substrates with to s-s bonds and cellular detoxification. DsbA-L is expressed mostly in adipose tissue, and its expression level is negatively correlated with the obesity of mice and humans.

DsbA-L has been shown to bind adiponectin and increase the production of the HMW species, for that it can become a novel therapeutic and industrial target.

1. A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin multimerization, Meilian Liua, Lijun Zhoub. 18302–18307PNAS ,November 25, 2008 vol. 105 no. 47.

2. Fat-Specific DsbA-L Overexpression Promotes Adiponectin Multimerization and Protects Mice From Diet-Induced Obesity and Insulin Resistance. Meilian Liu, Ruihua Xiang, DIABETES, VOL. 61, NOVEMBER 2012.

3. Role of the Endoplasmic Reticulum Chaperone DsbA-L Glutathione S-Transferase Activity in the Assembly of Adipocyte Hormone Adiponectin . Dr Tsu-Shuen Taso, kartchner, lavrel, Brianne, The University of Arizona library release, may 2011.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 508
  Illegal SapI site found at 27